Ludlam said it looks like a driver had run over two of the pots and one other driver said he thought he had seen two larger spiders on the loose.
"It appears someone ran over two of the pots and the driver told the woman who called us he thought he saw two larger spiders", RSPCA inspector Kristy Ludlam says of the unusual incident.
But locals are sure to be anxious as the spiders can grow to have a leg span of 10in.
An unsuspecting motorist knocked over a box of containers that housed individual Brazilian bird-eating tarantulas and their babies in Somercotes, near Alfreton.
While the missing tarantulas would not normally be expected to survive in the United Kingdom climate, warmer weather could mean that the spiders will stay alive for longer than expected. It is assumed they escaped'.
RSPCA inspector Kristy Ludlam said: 'The woman who contacted us was shaken as she is terrified of spiders.
GETTYWhen two ten-inch arachnids were spotted scuttling away the RSPCA was called
He found three baby spiders in the jars which he thinks are Brazilian bird-eating tarantulas. The RSPCA is warning people not to approach or try to handle any of the spiders - because that would be the first thing on everyone's minds - because they are thought to be from the Brazilian salmon pink bird-eating variety of huge spider and therefore are highly venomous.
The RSCPA, who believe the arachnids may have been abandoned, are keeping all the flowerpots warm as they predict that more eggs may be about to hatch.
Originally from Brazil, they have a venomous bite, but tend to stun their prey by flicking hairs from their abdomen which can cause pain and blindness if thrown into the eyes.
The owner of a nearby kennels and cattery said she was very anxious.
According to the Wildlife and Countryside Act 1981 it is an offence to release any non-native species into the wild. We're also going to be searching the area to make sure they're not here.
The rescued spiders have been taken to Arnold and Carlton veterinary centre in Nottingham where they will be cared for until they are ready to be rehomed, the RSPCA said.
Jeff Bezos becomes "richest in modern history" as Amazon workers strike
Mr Gates is now in second place with a worth of $95b, having given away 700 million Microsoft shares and $2.9b in cash since 1996. Bloomberg reported that Bezos has more wealth than anyone since at least 1982 when Forbes began publishing its wealth ranking.
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