Standing between the former World No.2 and a spot in her first final since winning her maiden major title will be Ashleigh Barty, the victor of all-unseeded affair when Camila Giorgi was forced to retire trailing 5-2 in the first set.
"The match showed two ends of the spectrum".
Konta did reach the final of the Nottingham Open last week but lost to world number 70 Donna Vekic.
On a decidedly cooler day in Birmingham, Johanna Konta was outhit by a determined Coco Vandeweghe in the final match of the day to decide the quarter-final line-ups.
But in a stunning performance against Barty, Kvitova battled back from losing the first set and stepped up in the second half of the decider to seal victory. "I think that is the most important thing", said the American. "The season is very long and I must look at the bigger picture", she said.
This was a chastening experience for Konta so close to the start of Wimbledon.
"I played Barty yesterday in doubles, so that gives me an idea, a little bit".
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Konta won only one point against Vandeweghe's first serve and appeared ill at ease throughout the match.
Former Wimbledon champion Pat Cash was courtside to watch Vandeweghe for the first time since becoming her coach. Ahead of Wimbledon, the world No 7 will try to work out what went wrong. "I had to get him down from commentating from Queen's", joked the 25-year-old New Yorker.
Second-seed Elina Svitolina of Ukraine was also knocked out at the Edgbaston Priory Club on Thursday after suffering a surprise three-set defeat by Italian qualifier Camila Giorgi.
After a dismal first set, in which she made 12 unforced errors compared to just one victor, Konta called on coach Wim Fissette, and there were signs early on that the on-court coaching had helped the fourth seed early on in the first set, though it eventually proved to have little effect as Vandeweghe just proved too strong.
"I could not have imagined a better comeback than I am having now", Kvitova said. "But for the moment it's very uncomfortable".
Kvitova will next play her fellow Czech Lucie Safarova, who survived a three-hour ordeal against Daria Gavrilova. She was forced to end it with the score, 4-6, 6-4.
Fifth seed Kristina Mladenovic edged into the quarter finals after a 6-4 7-6 win over China's Zhang Shuai.
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