Several remembrance events will be held throughout the day.
At around 2 a.m. June 12, Mateen opened fire on a crowded gay nightclub which was celebrating "Latin Night".
"I miss Pulse", she said.
"We will make sure the world is a better place because of our 49 angels", WKMG-TV quoted Orange County Mayor Teresa Jacobs as saying at the service before a performer sang "Over the Rainbow" to close the service as many on hand sobbed.
Last year's Tony Awards were held on the day of the shooting and it was acknowledged throughout show.
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Bacsinszky, who is about to turn 28, stated: "It's pretty amusing ". "I'm really happy to play here, it's an fantastic tournament. Ostapenko and Bacsinszky will play each other on Thursday, which happens to be the date of both competitors' birthdays.
She said the club, which has never reopened, will become a permanent memorial. But she said she is grateful for the outpouring of support.
The Pulse nightclub itself has since been surrounded by various tributes, and will now be turned into a permanent memorial. Pulse nightclub owner Barbara Poma and mayor Buddy Dyer are also scheduled to speak. Forty-nine people were killed inside the nightclub.
Toronto's Village will be holding a memorial tonight honouring 49 people shot dead during Orlando's Pulse nightclub massacre past year. A quarter of the 2.3 million inhabitants in the greater Orlando area are Hispanic, and half of those are of Puerto Rican heritage, according to the USA census bureau. Mateen was killed in the shootout.
Jeannine Williams used to live within walking distance of Pulse and was a frequent visitor. "Seeing this mural with the faces of the 49, seeing all these people here to support and remember and honor their lives, it's just so handsome".
As part of the governor's proclamation Scott has ordered that US and State of Florida flags be flown at half-staff until Monday at sunset at all state and local buildings, installations and grounds throughout Florida. The shooter, who declared his support for ISIL, was killed after a three-hour standoff with police officers. She is charged with obstruction and with aiding and abetting in federal court.
Church bells will toll throughout the Orlando area as residents reflect on the 49 patrons killed during a massacre at the gay nightclub Pulse in the worst mass shooting in recent US history.
The church bells at St. John's Episcopal Church tolled 49 times at noon to remember the lives of each one lost in the shooting.
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